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From: TSS ()
Subject: Efficient In Vitro Amplification of CWD PrPRES
Date: September 24, 2007 at 9:31 am PST

Efficient In Vitro Amplification of CWD PrPRES

Mon Sep 24, 2007
10:2871.248.128.51

Journal of Virology, September 2007, p. 9605-9608, Vol. 81, No. 17
0022-538X/07/$08.00+0 doi:10.1128/JVI.00635-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Efficient In Vitro Amplification of Chronic Wasting Disease PrPRES
Timothy D. Kurt,1 Matthew R. Perrott,1 Carol J. Wilusz,1 Jeffrey Wilusz,1
Surachai Supattapone,2 Glenn C. Telling,3 Mark D. Zabel,1 and Edward A.
Hoover1*
Department of Microbiology, Immunology, and Pathology, College of Veterinary
Medicine and Biomedical Sciences, Colorado State University, Fort Collins,
Colorado,1 Department of Biochemistry, Dartmouth Medical School, Hanover,
New Hampshire,2 Department of Molecular Biology and Genetics, University of
Kentucky, Lexington, Kentucky3

Received 25 March 2007/ Accepted 30 May 2007

Chronic wasting disease (CWD) of cervids is associated with conversion of
the normal cervid prion protein, PrPC, to a protease-resistant conformer,
PrPCWD. Here we report the use of both nondenaturing amplification and
protein-misfolding cyclic amplification (PMCA) to amplify PrPCWD in vitro.
Normal brains from deer, transgenic mice expressing cervid PrPC
[Tg(cerPrP)1536 mice], and ferrets supported amplification. PMCA using
normal Tg(cerPrP)1536 brains as the PrPC substrate produced >6.5 x 109-fold
amplification after six rounds. Highly efficient in vitro amplification of
PrPCWD is a significant step toward detection of PrPCWD in the body fluids
or excreta of CWD-susceptible species.

----------------------------------------------------------------------------
----
* Corresponding author. Mailing address: Department of Microbiology,
Immunology, and Pathology, College of Veterinary Medicine and Biomedical
Sciences, Colorado State University, Fort Collins, CO 80523. Phone: (970)
491-7587. Fax: (970) 491-0523. E-mail: edward.hoover@colostate.edu

Published ahead of print on 6 June 2007.

----------------------------------------------------------------------------
----
Journal of Virology, September 2007, p. 9605-9608, Vol. 81, No. 17
0022-538X/07/$08.00+0 doi:10.1128/JVI.00635-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

http://jvi.asm.org/cgi/content/abstract/81/17/9605

The potential impact of prion diseases on human health was greatly magnified
by the recognition that interspecies transfer of BSE to humans by beef
ingestion resulted in vCJD. While changes in animal feed constituents and
slaughter practices appear to have curtailed vCJD, there is concern that CWD
of free-ranging deer and elk in the U.S. might also cross the species
barrier. Thus, consuming venison could be a source of human prion disease.
Whether BSE and CWD represent intraspecies scrapie transfer or are newly
arisen prion diseases is unknown. Therefore, the possibility of transmission
of prion disease through other food animals cannot be ruled out. There is
evidence that vCJD can be transmitted through blood transfusion. There is
likely a pool of unknown size of asymptomatic individuals infected with
vCJD, and there may be asymptomatic individuals infected with the CWD
equivalent. These circumstances represent a potential threat to blood and
plasma supplies.


http://cdmrp.army.mil/nprp/nprpstatusrpt.doc


From: "Terry S. Singeltary Sr."
Subject: CWD UPDATE 88 AUGUST 31, 2007


http://lists.ifas.ufl.edu/cgi-bin/wa.exe?A2=ind0709&L=sanet-mg&T=0&P=450


Date: Wed, 29 Aug 2007 21:13:08 -0500
From: "Terry S. Singeltary Sr."
Subject: CWD NEW MEXICO RECORDS IT'S 19 CASE (near Texas border again)


http://lists.ifas.ufl.edu/cgi-bin/wa.exe?A2=ind0708&L=sanet-mg&T=0&P=26079

Monitoring the Potential Transmission of Chronic Wasting Disease to Humans
Using a Hunter Registry Database in Wyoming (405 lines)
From: Terry S. Singeltary Sr. <[log in to unmask]>
Date: Thu, 30 Aug 2007 21:23:42 -0500


http://lists.ifas.ufl.edu/cgi-bin/wa.exe?A2=ind0708&L=sanet-mg&T=0&F=&S=&P=27654


Subject: Cross-sequence transmission of sporadic Creutzfeldt-Jakob disease
creates a new prion strain

Date: August 25, 2007 at 12:42 pm PST


Subject: Cross-sequence transmission of sporadic Creutzfeldt-Jakob disease
creates a new prion strain
Date: August 25, 2007 at 12:42 pm PST

J Biol Chem. 2007 Aug 20; : 17709374


####################################

our results raise the possibility that CJD cases
classified as VV1 may include cases caused by
iatrogenic transmission of sCJD-MM1 prions or
food-borne infection by type 1 prions from animals,
e.g., chronic wasting disease prions in cervid. In fact,
two CJD-VV1 patients who hunted deer or
consumed venison have been reported (40, 41). The
results of the present study emphasize the need for
traceback studies and careful re-examination of the
biochemical properties of sCJD-VV1 prions.

###################################


REFERENCES...snip...end

FULL TEXT ;


http://www.jbc.org/cgi/content/abstract/M704597200v1?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=Cross-sequence+transmission+of+sporadic+Creutzfeldt-Jakob+disease+creates+a+new+&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT

http://lists.ifas.ufl.edu/cgi-bin/wa.exe?A2=ind0708&L=sanet-mg&T=0&P=21267

Re: Colorado Surveillance Program for Chronic Wasting Disease
Transmission to Humans (TWO SUSPECT CASES)


http://lists.ifas.ufl.edu/cgi-bin/wa.exe?A2=ind0704&L=sanet-mg&T=0&P=1165


The lecture tomorrow will coincide with the start of Prion 2007, the leading
scientific conference on prion diseases, which is being held in Edinburgh
until Thursday.

The aim of the lecture is to further public understanding of vCJD and
clarify the risk of human-to-human transmission posed by the thousands of
people who may be infected without showing any symptoms.

http://www.telegraph.co.uk/news/main.jhtml?xml=/news/2007/09/24/ncjd124.xml


SEE CJD BLOOD RECALLS TSS


http://www.google.com/search?hl=en&q=CJD+BLOOD+RECALLS+TSS&btnG=Search


http://search.yahoo.com/search?p=CJD+BLOOD+RECALLS+TSS&fr=yfp-t-501&toggle=1&cop=mss&ei=UTF-8

TSS




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