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From: TSS ()
Subject: Detection of PrPSc in Rectal Biopsy and Necropsy Samples from Sheep with Experimental Scrapie
Date: February 12, 2006 at 1:06 pm PST

Copyright © 2005 Published by Elsevier Ltd.
Detection of PrPSc in Rectal Biopsy and Necropsy Samples from Sheep with Experimental Scrapie

A. Espenesa, , C.McL. Pressa, T. Landsverka, M.A. Tranulisa, M. Aleksandersena, G. Gunnesa, S.L. Benestadb, R. Fuglestveita and M.J. Ulvunda

aNorwegian School of Veterinary Science, Oslo and Sandnes, Norway
bNational Veterinary Institute, Oslo, Norway

Received 11 August 2004; accepted 1 August 2005. Available online 7 February 2006.

Scrapie diagnosis is based on the demonstration of disease-associated prion protein (PrPSc) in brain or, in the live animal, in readily accessible peripheral lymphoid tissue. Lymphatic tissues present at the rectoanal line were readily obtained from sheep without the need for anaesthesia. The presence of PrPSc in such tissue was investigated in sheep infected orally with scrapie-infected brain material. The methods used consisted of immunohistochemistry and histoblotting on biopsy and post-mortem material. PrPSc was detected in animals with PrP genotypes associated with high susceptibility to scrapie from 10 months after infection, i.e., from about the time of appearance of early clinical signs. In the rectal mucosa, PrPSc was found in lymphoid follicles and in cells scattered in the lamina propria, often near and sometimes in the crypt epithelium. By Western blotting, PrPSc was detected in rectal biopsy samples of sheep with the PrP genotype VRQ/VRQ, after electrophoresis of material equivalent to 8 mg of tissue. This study indicated that rectal biopsy samples should prove useful for the diagnosis of scrapie in sheep.

Keywords: prion protein; rectal biopsy; scrapie diagnosis; sheep; viral infection

Correspondence to: A. Espenes, Department of Basic Sciences and Aquatic Medicine, P.O. Box 8146, dep.; N-0033 Oslo, Norway.


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